Flow Cytometric Immunophenotyping Assay
Immunophenotyping by flow cytometry is an acknowledged method for distinguishing between different cell populations like immune cells. Alfa Cytology provides a thorough and comprehensive flow cytometric immunophenotyping assay to characterize the diverse cell types involved in brain tumors, offering valuable insights into cell populations at a molecular level.
Overview of Flow Cytometric Immunophenotyping Assay
Immunophenotyping by flow cytometry is a potent method employed to analyze and profile cells based on the expression of particular proteins or markers on their surface or within the cell. By labeling cells with fluorescently labeled antibodies that bind to specific antigens, researchers can identify and quantify diverse cell types participating in the initiation, progression and metastasis of brain tumors. This technique plays a crucial role in the early detection of brain tumors, aiding in the classification of different types of brain tumors.
Fig.1 Representative gating strategy and tumor immune infiltrate analysis. (Tomaszewski WH., et al., 2021)
Our Services
Leveraging advanced flow cytometry techniques, Alfa Cytology provides finely crafted immunophenotyping services customized for brain tumor research. These services assist our clients in gaining deeper insights into the complex mechanisms driving tumor progression, ultimately facilitating the innovation of novel diagnostic strategies for brain tumors.
Homing Profile
By analyzing the expression of homing receptors and their corresponding ligands, we can detect the infiltration of immune cells and investigate how they migrate into the brain tumor microenvironment.
Activation State Analysis
The activation status of immune cells is discerned through the analysis of the expression levels of activation and inhibitory receptors, providing a comprehensive understanding of the immune cell response.
Cytokine Release Analysis
One of the most important characteristics of immune cells is to synthesize and secrete cytokines, which exert crucial effects on cellular signaling and immune response within the brain tumor microenvironment.
Applicable Cell Types
Possessed with cutting-edge flow cytometers and cell sorters integrated with a wide array of fluorochromes, we excel in the precise identification of various immune cell subpopulations. Leveraging our extensive expertise in immunophenotyping through flow cytometry, Alfa Cytology offers a comprehensive suite of cell immunophenotyping services tailored to brain tumor research.
Sample
Preparation
Instrument
Setup
Flow
Cytometry
Immunophenotypic
Analysis
Our Services Help You Achieve the Following Goals
- Quantify tumor infiltration and predict the aggressiveness of brain tumors.
- Assist in identifying novel tumor biomarkers and enhance the advancement of early brain tumor diagnostic strategies.
- Assess the efficacy of potential drugs and facilitate the development of innovative therapeutics for brain tumors.
Workflow of Flow Cytometric Immunophenotyping Assay
- Dendritic cells
- Neutrophils
Why Choose Us?
Customized
Solutions
In-depth
Analysis
Strong
Expertise
Short
Turnaround
It is essential to comprehensively characterize immune cells within the brain tumor microenvironment. Alfa Cytology offers a diverse range of services to thoroughly profile immune cells using advanced flow cytometry techniques. If you have more inquiries regarding our flow cytometric immunophenotyping assay services, please do not hesitate to reach out to our knowledgeable team to discuss and submit your specific requirements.
Reference
- Tomaszewski WH.; et al. (2021). Broad immunophenotyping of the murine brain tumor microenvironment[J]. Journal of immunological methods. 499, 113158.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
