GFAP Polyclonal Antibody

CAT#: NSC-P008

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Overview Specification

Target	GFAP
Cell Type	Radial glial cells, glial progenitors
Host	Rabbit
Isotype	IgG
Applications	WB, IHC, IF, ELISA
Reactivity	Human, mouse, rat, goat, hamster, pig, zebrafish
Conjugate	Unconjugated
Keywords	Radial glial cells, glial progenitors; Rabbit; IgG; WB, IHC, IF, ELISA

Product Information	It targets GFAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information	GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. Tissue specificity GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963). Involvement in disease Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function. Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. Isoforms Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes. Post-translational modifications Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963). Cellular localization GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.
Synonyms	GFAP
Full Name	Glial fibrillary acidic protein
Tested Reactivity	Human, mouse, rat
Cited Reactivity	Human, mouse, rat, goat, hamster, pig, zebrafish
Class	Polyclonal
Type	Antibody
Immunogen	GFAP fusion protein
Calculated Molecular Weight	432 aa, 50 kDa
Observed Molecular Weight	45-50 kDa
Gene ID	2670
GenBank Accession Number	BC013596
Gene Symbol	GFAP
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	Mouse brain tissue, U-251 cells
Positive IF Detected	Rat brain tissue

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