GLUT1 Polyclonal Antibody

CAT#: NSC-P009

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Overview Specification

Target	GLUT1
Cell Type	NSCs
Host	Rabbit
Isotype	IgG
Applications	WB, IHC, IF, FC, ChIP, ELISA
Reactivity	Human, mouse, rat, pig
Conjugate	Unconjugated
Keywords	NSCs; Rabbit; IgG; WB, IHC, IF, FC, ChIP, ELISA

Product Information	The immunogen of GLUT1 Fusion Protein expressed in E. coli.
Background Information	Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).What is the subcellular localization of GLUT1?Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.What molecules can be transported by GLUT1. The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate.What is the tissue expression pattern of GLUT1. GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.
Synonyms	DYT17, DYT18, GLUT, GLUT 1, GLUT1, HepG2 glucose transporter, PED, SLC2A1
Full Name	Solute carrier family 2 (facilitated glucose transporter), member 1
Tested Reactivity	Human, mouse, rat
Cited Reactivity	Human, mouse, rat, Lasiopodomys brandtii, pig
Class	Polyclonal
Type	Antibody
Immunogen	GLUT1 fusion protein
Calculated Molecular Weight	492 aa, 54 kDa
Observed Molecular Weight	45-55 kDa
Gene ID	6513
GenBank Accession Number	BC121804
Gene Symbol	SLC2A1
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	HT-29 cells, 37°C incubated mouse colon tissue
Positive IHC Detected	Rat brain tissue, human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue
Positive IF Detected	HeLa cells
Positive FC Detected	HeLa cells

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