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Human B7-H3 (4Ig)/B7-H3b Protein

Human B7-H3 (4Ig)/B7-H3b Protein

CAT#: ICP-A009

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Overview Specification

Species	Human
Tag	C-His-Avi
Expression Systems	Expi293
Keywords	Human; C-His-Avi; Expi293

Synonyms	B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Source	Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly27-Thr461.
Molecular Weight	The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin	Less than 1 EU per μg by the LAL method.
Purity	>95% as determined by Tris-Bis PAGE. >95% as determined by HPLC.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage	-20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution.
Notes	Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

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