Human B7-H3/CD276 Protein
CAT#: ICP-A007
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Overview
Specification
| Species
|
Human |
| Tag
|
C-His |
| Expression Systems
|
Expi293 |
| Keywords
|
Human; C-His; Expi293 |
| Synonyms
|
B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276 |
| Background
|
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amounts so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cell induction. |
| Source
|
Recombinant Human B7-H3/CD276 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu29-Pro245. |
| Molecular Weight
|
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| Endotoxin
|
Less than 1 EU per μg by the LAL method. |
| Purity
|
>95% as determined by Tris-Bis PAGE.
>95% as determined by HPLC. |
| Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution
|
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage
|
-20 to -80℃ for 12 months as supplied from the date of receipt.
-20 to -80℃ for 3-6 months in an unopened state after reconstitution.
2-8℃ for 2-7 days after reconstitution. |
| Notes
|
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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