Human B7-H4 Protein
CAT#: ICP-A008
Inquiry
Overview
Specification
| Species
|
Human |
| Tag
|
C-His-Avi |
| Expression Systems
|
Expi293 |
| Keywords
|
Human; C-His-Avi; Expi293 |
| Synonyms
|
B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291 |
| Background
|
B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status. |
| Source
|
Recombinant Human B7-H4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Phe29-Ala258. |
| Molecular Weight
|
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result. |
| Endotoxin
|
Less than 1 EU per μg by the LAL method. |
| Purity
|
>95% as determined by Tris-Bis PAGE.
>95% as determined by HPLC. |
| Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution
|
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage
|
-20 to -80℃ for 12 months as supplied from the date of receipt.
-20 to -80℃ for 3-6 months in an unopened state after reconstitution.
2-8℃ for 2-7 days after reconstitution. |
| Notes
|
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Related Products
