CAT#: ICP-A013
Species | Human |
Tag | C-His |
Expression Systems | Expi293 |
Keywords | Human; C-His; Expi293 |
Synonyms | CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
Background | CD47 (Cluster of Differentiation 47) also known as integrin-associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD-47 acts as a don't eat me the signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers. |
Source | Recombinant Human CD47 is expressed from Expi293 with His tag at the C-terminal. It contains Gln19-Pro139. |
Molecular Weight | The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1 EU per μg by the LAL method. |
Purity | >95% as determined by Tris-Bis PAGE. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage | -20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution. |
Notes | Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |