Human CD4/LEU3 Protein
CAT#: ICP-A012
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Overview
Specification
| Species
|
Human |
| Tag
|
C-His-Avi |
| Expression Systems
|
Expi293 |
| Keywords
|
Human; C-His-Avi; Expi293 |
| Synonyms
|
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
| Background
|
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Integral membrane glycoprotein plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule: peptide complex. |
| Source
|
Recombinant Human CD4/LEU3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys26-Trp390. |
| Molecular Weight
|
The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
| Endotoxin
|
Less than 1 EU per μg by the LAL method. |
| Purity
|
>95% as determined by Tris-Bis PAGE.
>95% as determined by HPLC. |
| Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution
|
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage
|
-20 to -80℃ for 12 months as supplied from the date of receipt.
-20 to -80℃ for 3-6 months in an unopened state after reconstitution.
2-8℃ for 2-7 days after reconstitution. |
| Notes
|
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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