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Human CNPY2 ELISA Kit

Human CNPY2 ELISA Kit

CAT#: NRE-K005

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Overview Specification

Sample Type	Serum, plasma, cell culture supernatants, cell lysates
Sensitivity	0.01 ng/mL
Range	0.156-10 ng/mL
Reactivity	Human
Keywords	Serum, plasma, cell culture supernatants, cell lysates; Human

Product Information	It is a solid-phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CNPY2 ELISA kit is to be used to detect and quantify protein levels of endogenous CNPY2. The assay recognizes human CNPY2. An antibody specific for CNPY2 has been pre-coated onto the microwells. The CNPY2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CNPY2 is added to detect the captured CNPY2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Background Information	CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth (PMID: 12826659). It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 is a HIF-1α regulated, secreted angiogenic growth factor that promotes smooth muscle cells migration, proliferation and tissue revascularization (PMID: 25589425). CNPY2 enhances cell spreading in fibroblasts and migration of rat C6 glioma cells (PMID: 15905959). CNPY2 was also shown to influence the expression of low-density lipoprotein receptor and FGF21 (PMID: 22378787). This kit is used to quantify CNPY2 level in vivo.
Synonyms	Canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9
Tests	1 x 96 well plate
Assay Type	Sandwich
Tested Applications	Sandwich ELISA
Gene ID	10330
Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

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