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Human ENO2 ELISA Kit

Human ENO2 ELISA Kit

CAT#: NRE-K006

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Overview Specification

Sample Type	Serum, plasma, cell culture supernatants
Sensitivity	0.11 ng/mL
Range	0.313-20 ng/mL
Reactivity	Human
Keywords	Serum, plasma, cell culture supernatants; Human

Product Information	It is a solid-phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ENO2 ELISA kit is to be used to detect and quantify protein levels of endogenous ENO2. The assay recognizes human ENO2. An antibody specific for ENO2 has been pre-coated onto the microwells. The ENO2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ENO2 is added to detect the captured ENO2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Background Information	ENO2, also named as Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
Synonyms	ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE
Tests	1 x 96 well plate
Assay Type	Sandwich
Tested Applications	Sandwich ELISA
Gene ID	2026
Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

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