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Human FAP Protein

Human FAP Protein

CAT#: CAR-T014

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Overview Specification

Species	Human
Tag	N-His
Expression Systems	Expi293
Keywords	Human; N-His; Expi293

Synonyms	FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Background	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Source	Recombinant Human FAP Protein is expressed from Expi293 with His tag at the N-terminal. It contains Leu26-Asp760.
Molecular Weight	The protein has a predicted MW of 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Endotoxin	Less than 1 EU per μg by the LAL method.
Purity	>95% as determined by Tris-Bis PAGE. >95% as determined by HPLC.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage	-20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution.
Notes	Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

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