Human HVEM/TNFRSF14 Protein

CAT#: ICP-A026

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Overview Specification
Species Human
Tag C-His
Expression Systems Expi293
Keywords Human; C-His; Expi293
Synonyms CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Background Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate positive signaling.
Source Recombinant Human HVEM/TNFRSF14 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu39-Val202.
Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.
Endotoxin Less than 1 EU per μg by the LAL method.
Purity >95% as determined by Tris-Bis PAGE.
>95% as determined by HPLC.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage -20 to -80℃ for 12 months as supplied from the date of receipt.
-20 to -80℃ for 3-6 months in an unopened state after reconstitution.
2-8℃ for 2-7 days after reconstitution.
Notes Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
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