CAT#: ICP-A026
Species | Human |
Tag | C-His |
Expression Systems | Expi293 |
Keywords | Human; C-His; Expi293 |
Synonyms | CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2 |
Background | Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate positive signaling. |
Source | Recombinant Human HVEM/TNFRSF14 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu39-Val202. |
Molecular Weight | The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1 EU per μg by the LAL method. |
Purity | >95% as determined by Tris-Bis PAGE. >95% as determined by HPLC. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage | -20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution. |
Notes | Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |