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Human LILRB2/CD85d/ILT4 Protein

Human LILRB2/CD85d/ILT4 Protein

CAT#: ICP-A005

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Overview Specification

Species	Human
Tag	C-His-Avi
Expression Systems	Expi293
Keywords	Human; C-His-Avi; Expi293

Synonyms	CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Background	The immunoglobulin-like transcript (ILT) comprises a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G, and HLA-F alleles.
Source	Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln22-His458.
Molecular Weight	The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
Endotoxin	Less than 1 EU per μg by the LAL method.
Purity	>95% as determined by Tris-Bis PAGE. >95% as determined by HPLC.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage	-20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution.
Notes	Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

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