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Human Nectin-4 Protein

Human Nectin-4 Protein

CAT#: ICP-A020

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Overview Specification

Species	Human
Tag	C-His-Avi
Expression Systems	Expi293
Keywords	Human; C-His-Avi; Expi293

Synonyms	EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specific interactions with NECTIN1.
Source	Recombinant Human Nectin-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly32-Val351.
Molecular Weight	The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
Endotoxin	Less than 1 EU per μg by the LAL method.
Purity	>95% as determined by Tris-Bis PAGE. >95% as determined by HPLC.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage	-20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution.
Notes	Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

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