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Human NKG2D/CD314 Protein

Human NKG2D/CD314 Protein

CAT#: CAR-T020

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Overview Specification

Species	Human
Tag	N-His-Avi
Expression Systems	Expi293
Keywords	Human; N-His-Avi; Expi293

Synonyms	CD314; D12S2489E; KLR; NKG2-D; NKG2D
Background	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells, and NKT cells.
Source	Recombinant Human NKG2D/CD314 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Phe78-Val216.
Molecular Weight	The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result.
Endotoxin	Less than 1 EU per μg by the LAL method.
Purity	>95% as determined by Tris-Bis PAGE.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage	-20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution.
Notes	Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

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