CAT#: ICP-A023
Species | Human |
Tag | C-His-Avi |
Expression Systems | Expi293 |
Keywords | Human; C-His-Avi; Expi293 |
Synonyms | CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue; TXGP1L; IMD16 |
Background | Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen-presenting cells but is following their activation. |
Source | Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu29-Ala216. |
Molecular Weight | The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1 EU per μg by the LAL method. |
Purity | >95% as determined by Tris-Bis PAGE. >95% as determined by HPLC. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage | -20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution. |
Notes | Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |