CAT#: ICP-A028
Species | Human |
Tag | C-His |
Expression Systems | Expi293 |
Keywords | Human; C-His; Expi293 |
Synonyms | CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
Background | B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
Source | Recombinant Human PD-L1/B7-H1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Phe19-Arg238. |
Molecular Weight | The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1 EU per μg by the LAL method. |
Purity | >95% as determined by Tris-Bis PAGE. >95% as determined by HPLC. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage | -20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution. |
Notes | Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |