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Human S100B ELISA Kit

Human S100B ELISA Kit

CAT#: NRE-K012

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Overview Specification

Sample Type	Serum, plasma, cell culture supernatants
Sensitivity	1 pg/mL
Range	15.6-1,000 pg/mL
Reactivity	Human
Keywords	Serum, plasma, cell culture supernatants; Human

Product Information	It is a solid-phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The S100B ELISA kit is to be used to detect and quantify protein levels of endogenous S100B. The assay recognizes human S100B. An antibody specific for S100B has been pre-coated onto the microwells. The S100B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for S100B is added to detect the captured S100B protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Background Information	S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain. It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte. Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage. An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD).
Synonyms	NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 calcium binding protein B, S100B, S100beta
Tests	1 x 96 well plate
Assay Type	Sandwich
Tested Applications	Sandwich ELISA
Gene ID	6285
Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

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