Human SIRP alpha/CD172a Protein
CAT#: ICP-A014
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Overview
Specification
| Species
|
Human |
| Tag
|
C-His |
| Expression Systems
|
Expi293 |
| Keywords
|
Human; C-His; Expi293 |
| Synonyms
|
CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA |
| Background
|
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from the SIRP family expressed mainly by myeloid cells and also by stem cells or neurons. SIRPα acts as an inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. |
| Source
|
Recombinant Human SIRP alpha/CD172a Protein is expressed from Expi293 with His tag at the C-terminal. It contains Glu31-Arg370. |
| Molecular Weight
|
The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
| Endotoxin
|
Less than 1 EU per μg by the LAL method. |
| Purity
|
>95% as determined by Tris-Bis PAGE.
>95% as determined by HPLC. |
| Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution
|
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage
|
-20 to -80℃ for 12 months as supplied from the date of receipt.
-20 to -80℃ for 3-6 months in an unopened state after reconstitution.
2-8℃ for 2-7 days after reconstitution. |
| Notes
|
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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