CAT#: ICP-A016
Species | Human |
Tag | C-His-Avi |
Expression Systems | Expi293 |
Keywords | Human; C-His-Avi; Expi293 |
Synonyms | CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2 |
Background | Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily. SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. |
Source | Recombinant Human SIRP Gamma/CD172g Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu29-Pro360. |
Molecular Weight | The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1 EU per μg by the LAL method. |
Purity | >95% as determined by Tris-Bis PAGE. >95% as determined by HPLC. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage | -20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution. |
Notes | Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |