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Human TDP-43 ELISA Kit

Human TDP-43 ELISA Kit

CAT#: NRE-K017

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Overview Specification

Sample Type	Human cerebrospinal fluid, cell lysates
Sensitivity	0.26 ng/mL
Range	6.25-400 ng/mL
Reactivity	Human
Keywords	Human cerebrospinal fluid, cell lysates; Human

Product Information	It is a solid-phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TDP-43 ELISA kit is to be used to detect and quantify protein levels of endogenous TDP-43. The assay recognizes human TDP-43. An antibody specific for TDP-43 has been pre-coated onto the microwells. The TDP-43 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TDP-43 is added to detect the captured TDP-43 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Background Information	TARDBP was firstly found as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene. It is used to quantify TDP-43 level in vivo and it is suitable for sample of CSF and cell lysates. It is used to quantify TDP-43 level in vivo and it is suitable for sample of serum or plasma.
Synonyms	ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Tests	1 x 96 well plate
Assay Type	Sandwich
Tested Applications	Sandwich ELISA
Gene ID	23435
Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

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