CAT#: ICP-A022
Species | Human |
Tag | C-His |
Expression Systems | Expi293 |
Keywords | Human; C-His; Expi293 |
Synonyms | TIGIT; VSIG9; VSTM3; WUCAM |
Background | TIGIT, also called Vstm3, Vsig9, and WUCAM is a transmembrane protein in the CD28 family of the Ig superfamily proteins. IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and are up-regulated following activation of these cells. |
Source | Recombinant Human TIGIT Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met22-Pro141. |
Molecular Weight | The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1 EU per μg by the LAL method. |
Purity | >95% as determined by Tris-Bis PAGE. >95% as determined by HPLC. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage | -20 to -80℃ for 12 months as supplied from the date of receipt. -20 to -80℃ for 3-6 months in an unopened state after reconstitution. 2-8℃ for 2-7 days after reconstitution. |
Notes | Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |