Human/Cynomolgus/Rhesus macaque CD28 Protein
CAT#: ICP-A011
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Overview
Specification
| Species
|
Human / Cynomolgus / Rhesus macaque |
| Tag
|
N-His-Avi |
| Expression Systems
|
Expi293 |
| Keywords
|
Human / Cynomolgus / Rhesus macaque; N-His-Avi; Expi293 |
| Synonyms
|
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 |
| Background
|
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
| Source
|
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Asn19-Pro152. |
| Molecular Weight
|
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result. |
| Endotoxin
|
Less than 1 EU per μg by the LAL method. |
| Purity
|
>95% as determined by Tris-Bis PAGE.
>95% as determined by HPLC. |
| Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution
|
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage
|
-20 to -80℃ for 12 months as supplied from the date of receipt.
-20 to -80℃ for 3-6 months in an unopened state after reconstitution.
2-8℃ for 2-7 days after reconstitution. |
| Notes
|
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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