Nestin Polyclonal Antibody

CAT#: NSC-P014

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Overview Specification
Target Nestin
Cell Type NSCs, radial glial cells, glial progenitors
Host Rabbit
Isotype IgG
Applications WB, IHC, IF, FC, ELISA
Reactivity Human, mouse, rat
Conjugate Unconjugated
Keywords NSCs, radial glial cells, glial progenitors; Rabbit; IgG; WB, IHC, IF, FC, ELISA
Product Information It targets Nestin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Synonyms NES, Nestin
Full Name Nestin
Tested Reactivity Human, mouse, rat
Cited Reactivity Human, mouse, rat
Class Polyclonal
Type Antibody
Immunogen Peptide
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 170-180 kDa
Gene ID 10763
GenBank Accession Number NM_006617
Gene Symbol NES
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected Mouse brain tissue, rat brain tissue
Positive IHC Detected Human kidney tissue, human gliomas tissue, mouse brain tissue
Positive IF Detected U-251 cells, rat brain tissue, SH-SY5Y cells, mouse brain tissue
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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