Nestin Polyclonal Antibody

CAT#: NSC-P014

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Overview Specification

Target	Nestin
Cell Type	NSCs, radial glial cells, glial progenitors
Host	Rabbit
Isotype	IgG
Applications	WB, IHC, IF, FC, ELISA
Reactivity	Human, mouse, rat
Conjugate	Unconjugated
Keywords	NSCs, radial glial cells, glial progenitors; Rabbit; IgG; WB, IHC, IF, FC, ELISA

Product Information	It targets Nestin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information	Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Synonyms	NES, Nestin
Full Name	Nestin
Tested Reactivity	Human, mouse, rat
Cited Reactivity	Human, mouse, rat
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Calculated Molecular Weight	177 kDa
Observed Molecular Weight	170-180 kDa
Gene ID	10763
GenBank Accession Number	NM_006617
Gene Symbol	NES
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	Mouse brain tissue, rat brain tissue
Positive IHC Detected	Human kidney tissue, human gliomas tissue, mouse brain tissue
Positive IF Detected	U-251 cells, rat brain tissue, SH-SY5Y cells, mouse brain tissue

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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