NF-L Polyclonal Antibody

CAT#: NEM-K006

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Overview Specification

Target	NF-L
Cell Type	Dopaminergic, glutamatergic, GABAergic neurons
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, FC, ELISA
Reactivity	Human, mouse, rat
Conjugate	Unconjugated
Keywords	Dopaminergic, glutamatergic, GABAergic neurons; Rabbit; IgG; WB, IP, IHC, IF, FC, ELISA

Product Information	It targets NF-L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information	NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.
Synonyms	68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L
Full Name	Neurofilament, light polypeptide
Tested Reactivity	Human, mouse, rat
Type	Antibody
Class	Polyclonal
Immunogen	NF-L fusion protein
Calculated Molecular Weight	543 aa, 62 kDa
Observed Molecular Weight	65-68 kDa, 140-160 kDa
GenBank Accession Number	BC039237
Gene ID	4747
Gene Symbol	NEFL
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	SH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells
Positive IP Detected	Mouse brain tissue
Positive IHC Detected	Mouse brain tissue
Positive IF Detected	SH-SY5Y cells
Positive FC Detected	SH-SY5Y cells

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