NOTCH1 Polyclonal Antibody

CAT#: NSC-P016

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Overview Specification

Target	NOTCH1
Cell Type	NSCs
Host	Rabbit
Isotype	IgG
Applications	WB, IHC, IF, FC, CoIP, ELISA
Reactivity	Human, mink, mouse, pig, rat, zebrafish
Conjugate	Unconjugated
Keywords	NSCs; Rabbit; IgG; WB, IHC, IF, FC, CoIP, ELISA

Product Information	It targets NOTCH1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
Background Information	NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV). Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 form (120 kDa).
Synonyms	hN1, Notch 1, NOTCH1, TAN1
Full Name	Notch homolog 1, translocation-associated (Drosophila)
Tested Reactivity	Human
Cited Reactivity	Human, mouse, rat, mink, pig, zebrafish
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Calculated Molecular Weight	273 kDa
Observed Molecular Weight	273-300 kDa, 120 kDa
Gene ID	4851
GenBank Accession Number	NM_017617
Gene Symbol	NOTCH1
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Positive WB Detected	HEK-293 cells, HeLa cells
Positive IHC Detected	Human breast cancer tissue, human lymphoma tissue, human ovary tumor tissue, mouse brain tissue

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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