| Product Information
|
It targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. |
| Background Information
|
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585). |
| Synonyms
|
VIM, vimentin |
| Full Name
|
Vimentin |
| Tested Reactivity
|
Human, mouse, rat |
| Cited Reactivity
|
Human, mouse, rat, bovine, deer, hamster, pig |
| Class
|
Polyclonal |
| Type
|
Antibody |
| Immunogen
|
Vimentin fusion protein |
| Calculated Molecular Weight
|
466 aa, 54 kDa |
| Observed Molecular Weight
|
54 kDa |
| Gene ID
|
7431 |
| GenBank Accession Number
|
BC000163 |
| Gene Symbol
|
VIM |
| Form
|
Liquid |
| Purification Method
|
Antigen affinity purification |
| Storage Buffer
|
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions
|
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Positive WB Detected
|
HeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue |
| Positive IHC Detected
|
Human liver cancer tissue, human ovary tumor tissue, human breast cancer tissue, human liver tissue, human tonsillitis tissue |
| Positive IF Detected
|
HepG2 cells, human breast cancer tissue, human kidney tissue |
| Positive FC Detected
|
HeLa cells |
