Vimentin Polyclonal Antibody

CAT#: NSC-P025

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Overview Specification

Target	Vimentin
Cell Type	Radial glial cells
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, FC, CoIP, ELISA
Reactivity	Human, mouse, rat, bovine, deer, hamster, pig
Conjugate	Unconjugated
Keywords	Radial glial cells; Rabbit; IgG; WB, IP, IHC, IF, FC, CoIP, ELISA

Product Information	It targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information	Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
Synonyms	VIM, vimentin
Full Name	Vimentin
Tested Reactivity	Human, mouse, rat
Cited Reactivity	Human, mouse, rat, bovine, deer, hamster, pig
Class	Polyclonal
Type	Antibody
Immunogen	Vimentin fusion protein
Calculated Molecular Weight	466 aa, 54 kDa
Observed Molecular Weight	54 kDa
Gene ID	7431
GenBank Accession Number	BC000163
Gene Symbol	VIM
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	HeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue
Positive IHC Detected	Human liver cancer tissue, human ovary tumor tissue, human breast cancer tissue, human liver tissue, human tonsillitis tissue
Positive IF Detected	HepG2 cells, human breast cancer tissue, human kidney tissue
Positive FC Detected	HeLa cells

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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