Orthotopic Tumor Model Development Services

The orthotopic tumor model is an experimental model in which a tumor is established in its natural location in the animal body, which can more realistically reflect the biological characteristics and microenvironment of the tumor. Alfa Cytology provides orthotopic tumor model development services to support tumor research and new drug development.

Introduction to Orthotopic Tumor Models

In situ transplantation tumor animal models are established by transplanting tumor cells into the organ of origin of that tumor, such as liver cancer cells/tissue transplanted into the liver or gastric cancer cells/tissue transplanted into the stomach. It provides the same in vivo microenvironment as the origin of the tumor cells, maintains the original biological properties of the tumor cells, has high clinical relevance, can accurately predict drug efficacy, and reduces the incidence of false positives. It is also possible to fully consider the role of the tumor microenvironment when studying tumor progression and treatment strategies, as well as to fully consider the interaction between tumor cells and their surrounding interstitial organ microenvironment when evaluating drug efficacy and monitoring tumor growth.

Fig.1 Comparison of the tumor microenvironment in preclinical murine models of orthotopic and ectopic triple-negative breast cancer. (Santana-Krímskaya, S. E., et al. 2022)

Advantages of Orthotopic Tumor Models

Orthotopic tumor models provide powerful support for oncology research because of their unique advantages, helping researchers to better understand tumor biology and its response to treatment.

Our Services

At Alfa Cytology, we have established a sophisticated tumor microenvironment center technology platform, a team of experienced tumor model customization experts, and proven technology to provide our global customers with efficient and proven in situ transplanted tumor models for tumor and microenvironment research and antitumor drug discovery.

Multiple Model Types

Multiple types of tumors in situ implants are offered for the development of customized tumor models to facilitate drug discovery projects.

Colorectal Cancer

• CT26 (mouse)
• HCT 116 (human)
• RKO (human)

Pancreatic Cancer

• MIA Paca-2 (human)

Breast Cancer

• 4T1 (mouse)
• MCF-1 (human)

Liver Cancer

• Hep 3B (human)

Ovarian Cancer

• ID8 (mouse)

Brain Cancer

• GL261 (mouse)

Kidney Cancer

• Renca (mouse)
• G401 (mouse)

Stomach Cancer

• Hs 746T (human)

Bladder Cancer

• MB49 (mouse)

Prostate Cancer

• LNCap Clone FGC (human)

Workflow of Orthotopic Tumor Model Development

With extensive experience in various cancer models and expertise in tumor and microenvironment, Alfa Cytology is the ideal partner to support your oncology therapeutic research. We provide customers with stock model products, model customization, and joint R&D services to provide you with the best tumor models and look forward to meeting your precise needs. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. If you have any questions, please feel free to contact us.

For research use only.