Metastatic Ovarian Cancer Model Development

A metastatic ovarian cancer model is a sophisticated experimental framework designed to study the process by which ovarian cancer spreads from the primary tumor site to other parts of the body. Alfa Cytology is dedicated to advancing ovarian cancer research and development, continuously pushing the frontiers in ovarian cancer metastasis modeling.

Introduction to Metastatic Ovarian Cancer Model

The metastatic ovarian cancer model provides a controlled environment to observe tumor growth, invasion, and metastasis by implanting human ovarian cancer cell lines, like SKOV-3 or OVCAR-3, into immunocompromised mice. Researchers can explore the molecular and cellular mechanisms driving metastasis and evaluate novel therapeutic strategies. Using bioluminescent imaging and histological analysis, these models elucidate tumor-host microenvironment interactions, offering a foundation for targeted therapy development.

Fig.1 The in vivo selection of highly metastatic (HM) OC cells. (YING F, et al., 2023)

Our Services

Focusing on cutting-edge cancer biology, Alfa Cytology has successfully developed an advanced ovarian cancer metastasis model. This model precisely simulates the metastatic process of ovarian cancer, paving the way for uncovering disease progression mechanisms and advancing the development of targeted therapies.

Workflow of Metastatic Ovarian Cancer Model

Cell Line and Animal Selection

According to the experimental requirements, our research team selects suitable ovarian cancer cell lines with high metastatic potential, and cultures and expands them under optimal conditions. We also select appropriate animal lines based on the specific experimental requirements and the chosen cell lines.

Model Construction

During the model construction stage, our research team selects the appropriate inoculation method according to the experimental requirements, such as transplanting cells into specific anatomical sites or directly inoculating them into the blood or lymphatic vessels. Successful model construction is verified through computed tomography (CT) imaging and pathological analysis.

Assessment of Metastatic Ability

To assess metastatic capacity, we monitors the number, size, and distribution of metastatic foci in post-metastatic organs (e.g., lungs, liver, or peritoneum) using in vivo imaging techniques or staining of H&E tissue sections. Additionally, proteins or biomarkers associated with metastasis (e.g., matrix metalloproteinases and cell adhesion molecules) are monitored to explore metastatic mechanisms in depth.

Ovarian Cancer Metastatic Model

Model	Measure
Peritoneal Metastasis	Our research team injects ovarian cancer cells into the peritoneal cavity to simulate the process of spreading and metastasis of cancer cells to the peritoneum.
Lymph Node Metastasis	We also implant cancer cells into the ovaries or other related tissues to observe the process of cancer cell metastasis to the lymph nodes.
Liver Metastasis	To characterize the metastasis of ovarian cancer cells to the liver, our research team injects the cells into the bloodstream of mice.
Lung Metastasis	By injecting ovarian cancer cells intravenously, we can observe their metastasis and growth in the lungs.
Bone Metastasis	We implant ovarian cancer cells into the bone tissue of mice to study the mechanism and characteristics of bone metastasis.

Alfa Cytology offers a comprehensive, one-stop service for constructing ovarian cancer metastasis models. Our team of experts is equipped with the experience and expertise to support you throughout every stage of your research, from cell culture to model optimization, ensuring seamless progress and reliable results. If you have any questions about our services or encounter any related issues, please feel free to contact us.

Reference

YING F, GUO J, GAO X, et al. Establishment of highly metastatic ovarian cancer model with omental tropism via in vivo selection [J]. iScience, 2023, 26(5): 106719.

! For research use only.