Organoids Models Development

The ovarian cancer organoid model represents a cutting-edge in vitro culture technology that more accurately replicates the cellular interactions and tumor growth dynamics of ovarian cancer. Alfa Cytology brings extensive research expertise to this field, leading advancements in ovarian cancer organoid model development.

Introduction to Organoids Models of Ovarian Cancer

The ovarian cancer organoid model is an advanced 3D cell culture system that simulates the tissue structures and microenvironment of ovarian cancer. By co-culturing cancer cells with matrix materials, these organoids reproduce the growth patterns and interactions of cancer cells with their surroundings. This model replicates key tumor characteristics like cell proliferation, invasion, and metastasis, providing a robust platform for studying tumorigenesis, evaluating drug efficacy, and exploring personalized treatments.

Fig. 1 General overview for OC organoids. (YANG J, et al., 2021)

Cultivation of Organoid Models

Our Services

Alfa Cytology demonstrates exceptional expertise in ovarian cancer organoid models and is dedicated to advancing personalized treatment. Utilizing cutting-edge technologies, they replicate the biological characteristics of ovarian cancer, facilitating high-throughput drug screening and mechanism research.

Workflow of Organoids Models Development

Cell Culture and Expansion

The collected cells are cultured and expanded under specific conditions to obtain a sufficient number of cells for further experimentation.

Organoid Construction

Expanded cells are transformed into organoids using three-dimensional culture techniques. The culture medium and growth factors are adjusted to mimic the tumor microenvironment accurately.

Characterization and Validation

The generated organoids are characterized and validated through morphological, gene expression, and functional analyses to confirm their similarity to the original tumor.

Pharmacodynamic Evaluation

Different drug treatments are applied to the organoids to assess their effects on cell proliferation and survival. The drug effects are quantified using MTT and CCK-8 assays to analyze the inhibitory impacts of the drugs on the organoids.

Application for Organoids Models of Ovarian Cancer

Modeling Carcinogenesis and Progression

By simulating cancer cell-tissue interactions, we gain insights into tumor biology, metastasis, and the mechanisms of tumor invasion and spread.

• Building Organoids
• Recreating Tumor Microenvironment
• Tumor Characterization

Drug Screening

We use ovarian cancer organoid models to assess drug efficacy and safety in the real tumor microenvironment.

• High-Throughput Drug Testing
• Assessing Drug Sensitivity
• Studying Drug Combinations in Therapy

Organoid Biobank

Our organoid biobank stores living ovarian cancer organoids of various subtypes and grades. These enable precise drug assessment and biomarker identification.

• Sample Storage & Management
• Drug Screening Platform
• Mechanistic Research

Organoid-on-a-Chip

We integrate ovarian cancer organoids with microarray technology to replicate the tumor microenvironment in miniature in vitro systems.

• Biomarker Discovery
• Gene Function Studies
• Metabolic Profiling

With proven expertise in researching ovarian cancer organoid models, Alfa Cytology helps the clients to gain insights into the biological characteristics of ovarian cancer and supports drug screening. If you have any questions about our ovarian cancer organoid model services, please feel free to contact us.

! For research use only.