Orthotopic Ovarian Cancer Model Development

Orthotopic ovarian cancer models are essential experimental tools specifically designed to enable an in-depth study of ovarian cancer onset, progression, and response to treatment. Alfa Cytology brings many years of extensive research experience in this field and is committed to developing and optimizing orthotopic cancer models to advance ovarian cancer research.

Introduction to Orthotopic Ovarian Cancer Model

The orthotopic ovarian cancer model simulates tumor growth in its natural microenvironment by implanting human ovarian cancer cells into the ovaries of mice or other experimental animals. This model reflects ovarian cancer's biological characteristics and the tumor-host interactions. Researchers can observe tumor growth, metastasis, and interactions with surrounding tissues, as well as evaluate new therapies' efficacy and safety, thereby advancing precision treatment research for ovarian cancer.

Showing the establishment process of PDX. Fig.1 Schematic diagram of the construction of an orthotopic ovarian cancer model.

Animal Species

Species	Advantages
Mice	High genetic homology to humans, low cost, easy rearing, high survival, stable genetic traits, and the formation of various inbred strains enable repeatable experiments.
Rats	Similar genetic sequence and genome to humans, disease resistance, adaptability, high survival rates, and larger organs and tissues facilitate observation and statistics.
Other Animals	Rabbits and guinea pigs may also be considered depending on the experiment's purpose, the animal's biology, and feasibility.

Our Services

Alfa Cytology is dedicated to developing orthotopic ovarian cancer models that enhance the accuracy and validity of ovarian cancer research, contributing to a deeper understanding of tumor biology and the tumor microenvironment. This effort promotes advancements in precision therapy.

Workflow of Orthotopic Ovarian Cancer Model Development

Tumor Implantation

After anesthetizing the animal, perform surgery to implant the cancer cells directly into the ovary or the abdominal cavity. Ensure that the cells are maintained in a suitable environment that accurately mimics the natural growth pattern of ovarian tumors.

Suitable Cell Lines or Primary Cells

Select an appropriate ovarian cancer cell line (e.g., OVCAR, SKOV3, etc.) or primary cells derived from tumor tissues to ensure relevance and suitability for the model.

Efficacy Assessment

Use the orthotopic ovarian cancer model to conduct drug therapy experiments. Assess the efficacy and side effects of different treatment regimens to identify potential therapeutic agents and optimize treatment strategies.

Evaluation of Tumor Characteristics

Perform histological analysis and molecular biology tests to assess tumor biomarkers, the proliferation index, and other relevant characteristics to validate the model.

Cell Lines in Orthotopic Ovarian Cancer Model

Alfa Cytology provides a variety of cell lines for the construction of orthotopic ovarian cancer models.

OVCAR-3: A highly differentiated human ovarian cancer cell line, widely utilized for studying ovarian cancer's biological properties and drug sensitivity.
SKOV-3: Another commonly used human ovarian cancer cell line, known for its high metastatic potential, suitable for metastasis studies.
HeyA8: Ideal for studying ovarian cancer cell survival mechanisms related to chemotherapy.
COV362: Suitable for investigating the interaction between the tumor microenvironment and ovarian cancer.

Equipped with state-of-the-art research instruments and an exceptional research team, Alfa Cytology is dedicated to assisting you with your most challenging research projects. Our team brings extensive expertise across various domains of biological sciences, ensuring that your research is conducted with the utmost precision and innovation. If you have any questions about our comprehensive range of services, please do not hesitate to contact us.

! For research use only.