Patient-Derived Xenograft (PDX) Model Development

The patient-derived xenograft (PDX) model is a state-of-the-art research tool that offers a unique platform for studying tumor growth and progression by transplanting patient tumor tissue directly into immunodeficient mice. Alfa Cytology is dedicated to developing a PDX model for ovarian cancer, an innovative approach that promises to significantly advance ovarian cancer research.

Introduction to Patient-Derived Xenograft (PDX) Model

The PDX model for ovarian cancer is a cutting-edge research tool that accurately replicates human tumor growth and progression. By transplanting tumor tissues from patients into immunodeficient mice, this model preserves the tumor's unique histological and genetic features. PDX models offer researchers critical insights into tumor development, metastasis, and drug resistance by maintaining the complexity and heterogeneity of the original tumors.

Fig.1 Showing the establishment process of PDX. (LIU Y, et al., 2023)

Application of PDX Model in Ovarian Cancer

• Personalized Medicine: Offers a closer representation of patient-specific tumor biology, enabling tailored therapy.
• Drug Screening: Enables drug efficacy and mechanism testing in a human-like tumor environment.
• Biomarker Discovery: Facilitates the identification of novel biomarkers and therapeutic targets.
• Resistance Studies: Allows investigation into drug resistance mechanisms and potential overcome strategies.

Our Services

Dedicated to the development of PDX models for ovarian cancer, Alfa Cytology aims to furnish the clients with a powerful tool for advancing ovarian cancer research. These models accurately simulate human tumor growth and metastasis, enabling researchers to gain a deeper understanding of the molecular mechanisms underpinning ovarian cancer.

Workflow of PDX Model Development

Sample Collection

Tumor tissue samples are obtained from ovarian cancer individual undergoing surgical treatment. These samples, usually derived from primary ovarian tumors, are collected in a way that avoids prior chemotherapy as much as possible to preserve the tumor's original characteristics.

Transplantation Procedure

Small pieces of the collected tumor tissue are implanted into immunodeficient mice (e.g., NOD-SCID or NSG mice). These mice can accept the growth of human tumor cells due to their lack of an effective immune system.

Tumor Growth and Monitoring

After transplantation, researchers conduct regular monitoring of tumor growth in the mice, meticulously recording changes in tumor volume. This allows for the assessment of proliferative capacity and detailed observation of biological behavior over time.

Therapeutic Evaluation

The PDX model is particularly useful for evaluating the effectiveness of new drugs or treatment regimens. Researchers can apply different drug treatments to the mice and observe the tumor's response, including tumor shrinkage, stability, or progression.

Ovarian Cancer PDX Model

Alfa Cytology leverages state-of-the-art instrumentation and an exceptional research team to continuously refine and advance the development of PDX models for ovarian cancer. For any inquiries or further information about our services, please do not hesitate to contact us.

! For research use only.