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Project Description

Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

PDX Model Development Services

Alfa Cytology specializes in comprehensive patient-derived xenograft (PDX) model development services. We are committed to creating robust, reliable PDX models, helping researchers around the world streamline the drug development process.

Introduction to Patient-Derived Xenograft (PDX) Model

Patient-derived tumor xenograft models (PDX) are tumor models created by transplanting fresh tumor tissue from a patient directly into an immunodeficient mouse, which allows tumors to grow in living organisms while maintaining the original histological and genetic characteristics of the patient's tumor. The PDX model does not require artificial culture and maintains the heterogeneity of the tumor, resulting in a more complete biology and the ability to accurately replicate the human tumor microenvironment, which is the best tumor animal model at this stage.

Fig. 1 Humanized patient tumor xenograft (PDX) model construction protocol. Fig.1 Protocols of humanized patient-derived tumor xenografts (PDX) model construction. (Zhang, W., & Zheng, X., 2023)

Applications and Research Trends of PDX Model

Patient-derived tumor xenografts (PDX), as a classical in vivo experimental method, are widely used in all stages of drug development, from target validation and lead compound optimization to preclinical efficacy and safety studies. Recent advances in PDX model development include the creation of humanized PDX models, where mice are engrafted with human hematopoietic stem cells to reconstitute a human immune system. This innovation has expanded the utility of PDX models in immuno-oncology.

Our Services

Alfa Cytology offers a wide range of patient-derived xenograft (PDX) models for a variety of cancer types, designed to support preclinical and translational research in cutting-edge cancer therapies. We have more than 3,000 models in place, and these models are continuously updated with the latest research hotspots.

Patient-Derived Xenograft (PDX) Models Available

Alfa Cytology can provide you with PDX models for a wide range of cancers such as acute myeloid leukemia, adrenal cancer, bladder cancer, brain cancer, breast cancer, cervical cancer, bile duct cancer, colorectal cancer, esophageal cancer, gallbladder cancer, gastric cancer, head and neck cancer, renal cancer, liver cancer, lung cancer, lymphoma, melanoma, mesothelioma, mixed Müllerian duct cancer, ovarian cancer, pancreatic cancer, prostate cancer, sarcoma, testicular cancer, thyroid cancer, uterus cancer and many more.

Type of Cancer	PDX Models Available
Breast Cancer	Triple-negative breast cancer (TNBC) models; HER2-positive breast cancer models; Hormone receptor-positive (ER/PR+) breast cancer models.
Lung Cancer	Non-small cell lung cancer (NSCLC) models with EGFR mutations; Small cell lung cancer (SCLC) models; NSCLC models with KRAS mutations.
Colorectal Cancer	KRAS-mutant colorectal cancer models; BRAF-mutant colorectal cancer models; MSI-H colorectal cancer models.
Pancreatic Cancer	Pancreatic ductal adenocarcinoma models; Pancreatic neuroendocrine tumor models; PDX models with SMAD4 and TP53 mutations.
Prostate Cancer	Hormone-sensitive prostate cancer models; Castration-resistant prostate cancer (CRPC) models; PDX models with PTEN loss.
Ovarian Cancer	High-grade serous ovarian carcinoma (HGSOC) models; Clear cell ovarian cancer models; Endometrioid ovarian cancer models.
Glioblastoma	EGFR-amplified glioblastoma models; MGMT promoter methylated glioblastoma models; IDH1-mutant glioblastoma models.
Melanoma	BRAF V600E mutant melanoma models; NRAS mutant melanoma models; Wild-type melanoma models.
Liver Cancer	Hepatocellular carcinoma (HCC) models; Intrahepatic cholangiocarcinoma models; Fibrolamellar hepatocellular carcinoma models.
Esophageal Cancer	Esophageal squamous cell carcinoma (ESCC) models; Esophageal adenocarcinoma models; PDX models with HER2 amplification.

Advantages of Our PDX Models

With our rigorous standardized procedures, we custom develop PDX models with a high degree of accuracy and relevance to aid in the study of human cancer biology, increasing the translational potential of research results.

Retention of Tumor Heterogeneity

Our PDX models retain the original patient's tumor's histological and genetic diversity, ensuring accurate representation of intra-tumoral heterogeneity.

Customization and Scalability

PDX models are customizable and scalable to fit specific research needs, enabling tailored experiments and high-throughput drug screening.

Human-like Tumor Microenvironment

Our models preserve the human tumor microenvironment, including stromal cells and extracellular matrix, facilitating the study of tumor-stroma interactions.

Compatibility with Advanced Research Techniques

These models are compatible with next-generation sequencing, immunohistochemistry, and in vivo imaging, allowing for comprehensive molecular and cellular analyses.

With extensive experience in various cancer models and expertise in a wide range of cancer modeling, Alfa Cytology is the ideal partner to support your oncology therapeutic research. We provide customers with stock model products, model customization, and joint R&D services to provide you with the best tumor models and look forward to meeting your precise needs. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. For further inquiries and custom model development, please feel free to contact us.

Reference

Zhang, W., & Zheng, X. (2023). Patient-derived xenografts or organoids in the discovery of traditional and self-assembled drug for tumor immunotherapy. Frontiers in Oncology, 13, 1122322.

For research use only.