Cell Line-Derived Xenograft (CDX) Model Development

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Cell Line-Derived Xenograft (CDX) Model Development

Cell line-derived xenograft (CDX) models, although lacking heterogeneity, are a bridge between in vitro and in vivo studies. At Alfa Cytology, we use rigorous standards to provide a one-stop for cell line-derived xenograft (CDX) development models to ensure that they truly reflect the clinical features of prostate cancer.

Introduction to Cell Line-Derived Xenograft Model (CDX)

Cell line-derived xenograft, (CDX) model is a tumor model constructed by transplanting in vitro cultured human-derived tumor cell lines into immunodeficient mice. This model is currently one of the most commonly used in vivo models for preclinical pharmacodynamic evaluation of antitumor drugs, and is in great demand in prostate cancer research and antitumor drug development.

Features of Cell Line-Derived Xenograft (CDX) Model

Cell line-derived xenograft (CDX) models are characterized by the following main features.

Advantages Disadvantages
  • Suitability for Mechanism Studies: CDX models are well-suited for studying the mechanisms of tumor growth and response to treatments.
  • Rapid Growth: These models exhibit rapid tumor growth, which is beneficial for quick assessment of therapeutic effects.
  • Evaluation of Cytotoxic Agents: CDX models are effective for evaluating non-targeted cytotoxic agents.
  • Availability and Cost-Efficiency: They are widely available and cost-efficient to generate, making them accessible for various research purposes.
  • Lack of Heterogeneity: Compared to the original tumors, CDX models display low heterogeneity, which may affect the representativeness of the tumor's biology.
  • Absence of Immunological Context: Since CDXs are established in immune-deficient mice, they lack the immunological context, limiting their use in the study of immunotherapies and immunomodulatory drugs.
  • Lack of Tumor Micro-Environment: CDX models do not fully replicate the tumor micro-environment, which is crucial for understanding tumor biology and drug response.

Our Services

At Alfa Cytology, we have a proven platform and a dedicated team of professionals to customize the most appropriate CDX tumor models according to the research objectives and needs. We can inoculate cells subcutaneously or in situ in mice or rats, and can also use our humanized mouse models to evaluate immunotherapies within the intact human immune system.

Workflow for Cell Line-Derived Xenograft (CDX) Model Development

Feasibility Studies

Cell Line Selection

Through rigorous screening and characterization, we ensure the authenticity, stability and genetic fidelity of the selected cell lines. The cell lines mainly used to construct CDX models were PC-3, DU-145, LNCaP, C4-2, 22RV-1, Vcap.

Optimization

Inoculation Method and Animal Selection

Depending on your research needs and target selection, we offer subcutaneous implantation, in situ transplantation, subperitoneal transplantation, intravascular inoculation transplantation, etc. We also provide you with the most appropriate inoculated mouse strains, such as nude mice, SCID mice, NOD SCID mice, NOD mice or NSG mice, to ensure optimal tumor transplantation and growth results.

Preclinical Studies

Model Characterization

After finalizing the model, we'll inoculate tumor cells and monitor growth with advanced techniques, assessing development and metastasis in real time for model accuracy. We'll also conduct comprehensive evaluations using histopathology, immunohistochemistry, genetic profiling, and molecular biomarkers to ensure the model's scientific validity.

Professionalism Optimization

Efficacy Evaluation

Offer comprehensive efficacy evaluation, testing new or existing treatments, and comparing tumor growth in treated vs. control groups to evaluate drug efficacy. This can help you understand how a drug works and speed up the drug development process.

At Alfa Cytology, we are committed to providing you with a comprehensive and efficient prostate cancer tumor model development service to support your research and drug development needs. If you are interested in our services, please don't hesitate to contact us for further information and pricing details.

notification For research use only.

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