In Vitro Modeling Services for Prostate Cancer

In vitro models are indispensable for prostate cancer drug research; they replicate the tumor's microenvironment and accelerate the preclinical phase of efficiently testing drug effects. At Alfa Cytology, we provide our clients with comprehensive In vitro modeling services for prostate cancer.

Introduction to In Vitro Modeling

In vitro models are critical in prostate cancer drug development. In vitro models of the prostate are able to mimic the biological behavior of tumors in a controlled laboratory environment, thereby accelerating drug screening, reducing costs, and increasing the efficiency of research.

The following table summarizes the advantages, limitations, and applications of different in vitro prostate cancer models.

Table 1. Comparison between simple and complicated prostate cancer (PCa) models in vitro. (Kedi Y., et al. 2024)

In vitro PCa models	Advantages	Limitations	Applications
2D cell lines	Easy accessibility, simple cultivation Stable reproducibility Low cost	Lack of 3D organization Loss of characteristics during passage	Tumor heterogeneity reflection Drug sensitivity assays
3D tumor spheroids	3D cancer cell growth High throughput	Disability of self-organization Monotypic cells	Cell origin detection Drug sensitivity assays
Patient-derived organoids	3D cancer cell growth Self-organization capacity Genetic heterogeneity recapitulation	Low success rate of establishment Absence of TME	Cell origin study Genetic mutation detection Drug screening
2D co-culture	Coexistence of cancerous and non-cancerous cells Convenience and ease	Absence of spatial structure Inaccurate genetic alternation False drug sensitivity results	Direct contact interaction between cells Soluble factor's function Extracellular matrix modelling Drug sensitivity assays
Spheroids-based co-culture	3D interaction between heterotypic cells Stable accessibility and cost effectiveness	Disability of self-organization Lack of genetic heterogeneity in the model	3D extracellular matrix modelling Stromal and immune microenvironment modelling Bone metastatic environment modelling Drug sensitivity assays
Organoids-based co-culture	Modelling heterogenic tumors with the presence of other TME components Self-organization capacity Tumorigenesis recapitulation Reliable drug screening results	Low success rate of establishment Difficult passage High expense	Extracellular matrix study Stromal microenvironment modelling Precision medicine
Microfluidic-based system	Modelling physiological factors Mimicking peripheral circulation	High expense	Physiological factor's function High-throughput drug screening
Tissue slice culture	Local microenvironment preservation Patient characteristics maintenance Feasible for spatial transcriptomic study	Short viable time Difficult to study cell interactions Low reproducibility	Drug sensitivity assay Spatial transcriptomic analysis
ALI-PDO	Co-culture of epithelial and non-epithelial cell populations Tumorigenesis recapitulation	Low success rate of establishment Difficult passage	TME modelling Drug screening Precision medicine
Organoids-on-a-chip	Incoporating pathophysiological factors Modelling interactions between organs	The low success rate of establishment High expense	High-throughput drug screening Precision medicine

Our Services

At Alfa Cytology, leveraging our expertise, cutting-edge technology, and dedication to scientific rigor, we are committed to aiding researchers and pharmaceutical enterprises in their pursuit of improved diagnostics, innovative therapies, and tailored prostate cancer treatment strategies through our advanced in vitro prostate cancer modeling services.

In vitro 2D models

Primary Cell Line
iPS Cells
RISPR Knockout Cell Lines
Knockdown Cell Lines
Knockin Cell Lines
Overexpression Cell Lines

In vitro 3D models

Alfa Cytology offers sophisticated in vitro prostate cancer modeling services, propelling research and development forward with a comprehensive and state-of-the-art methodology. We guarantee that your research endeavors are equipped with superior, dependable In vitro prostate cancer models. If you are interested in our services, please don't hesitate to contact us for further information and pricing details.

Reference

Kedi, Y., et al. Modelling the tumor microenvironment in vitro in prostate cancer: current and future perspectives. VIEW. 2024.

For research use only.

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In Vitro Modeling Services for Prostate Cancer

Introduction to In Vitro Modeling

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